QIAamp DNA Stool Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction was performed according to the instructions of the manufacturer (QIAGEN, Hilden, Germany) with the following modifications: 100 mg fecal sample was mixed with 1.4 mL ASL buffer in a 2 mL tube and vortexed until the sample was thoroughly homogenized. Samples were subsequently mixed with 0.2 g sterile zirconia/silica beads (diameter, 0.1 mm; Biospec Product, ROTH, Karlsruhe, Germany). Hereafter, the samples were processed on a TissueLyser (Qiagen Retsch GmbH, Hannover, Germany) for 6 minutes at 30 Hz [16,17]. Lysis was completed at a temperature of 95°C for 5 minutes. Finally, DNA was extracted according to the instruction of the QIAamp DNA stool MiniKit and eluted in 100 μL elution buffer provided in the kit.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Stool Mini Kit below.

Download manufacturer protocol

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