QIAamp DNA Stool Mini Kit

DNA isolation / purification Bacteria - Gram positive Lactobacillus

Experiment
DNA isolation / purification Bacteria - Gram positive Lactobacillus
Product
QIAamp DNA Stool Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction was performed according to the instructions of the manufacturer (QIAGEN, Hilden, Germany) with the following modifications: 100 mg fecal sample was mixed with 1.4 mL ASL buffer in a 2 mL tube and vortexed until the sample was thoroughly homogenized. Samples were subsequently mixed with 0.2 g sterile zirconia/silica beads (diameter, 0.1 mm; Biospec Product, ROTH, Karlsruhe, Germany). Hereafter, the samples were processed on a TissueLyser (Qiagen Retsch GmbH, Hannover, Germany) for 6 minutes at 30 Hz [16,17]. Lysis was completed at a temperature of 95°C for 5 minutes. Finally, DNA was extracted according to the instruction of the QIAamp DNA stool MiniKit and eluted in 100 μL elution buffer provided in the kit.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Stool Mini Kit below.

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Videos

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