RNAGEM™ Tissue PLUS
RNA isolation / purification Tissue - Rat Pineal gland
Protocol tips
| Downstream tips |
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| - Absorbance 260/280 nm is an ineffective quantitation method with RNAGEM-prepared nucleic acids |
Publication protocol
For qRT-PCR analysis of the effect of Crx-sh on Aanat mRNA levels in cultured rat pinealocytes, pinealocyte samples were centrifuged and total RNA was extracted using RNAGEMM Tissue (ZyGEM) according to the instruction of the manufacturer.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from ZyGEM for RNAGEM™ Tissue PLUS below.
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