RNeasy Mini Kit

RNA isolation / purification Tissue - Rat Placenta

Experiment

RNA isolation / purification Tissue - Rat Placenta

Product

RNeasy Mini Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Six samples of total RNA from the anterior pituitary, including three immature pituitary samples and three mature pituitary samples, were extracted using the TRIzol reagent (Tiangen, Beijing, China) according to the manufacturer’s recommended protocol, and the RNA quantity was assessed with a NanoDrop ND-2000 spectrophotometer (NanoDrop Technologies). After testing, the three immature and the three mature total RNA samples of the anterior pituitary were combined into their respective groups to construct the immature and mature anterior pituitary libraries, and the main processes for constructing the two libraries were as follows.

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Placenta using RNeasy Mini Kit from Qiagen.

Paper title

Mechanism of nucleoside uptake in rat placenta and induction of placental CNT2 in experimental diabetes.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download manufacturer protocol

Videos

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