Wizard® Genomic DNA Purification Kit

DNA isolation / purification Bacteria - Gram negative E.coli

Experiment
DNA isolation / purification Bacteria - Gram negative E.coli
Product
Wizard® Genomic DNA Purification Kit from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

Microbiological and molecular identification:samples for E. coli isolation were seeded and cultured in MacConkey agar by the streaking culture technique and incubated at 37 °C for 24 hours in aerobiosis. Samples for Campylobacter were suspended in 1 mL of saline solution and inoculated into a cellulose filter (0.45 µm) on blood agar and then incubated for 72 hours at 42 °C under microaerophilic conditions22. According to biochemical patterns and modified Gram staining with fuchsin, E. coliand Campylobacter were presumptive identified respectively34. The extraction of genomic DNA of each colony was performed by the kit Wizard Genomic DNA Purification for Gram-negative bacteria, according to the supplier's instructions (Promega, USA).

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative E.coli using Wizard® Genomic DNA Purification Kit from Promega.

Paper title
ISOLATION AND MOLECULAR IDENTIFICATION OF POTENTIALLY PATHOGENIC AND IN FERAL PIGEONS FROM AN URBAN AREA IN THE CITY OF LIMA, PERU
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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

Download PDF Download manufacturer protocol

Videos

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