Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not centrifuge Filter Cartridges at relative centrifugal forces greater than 10,000 x g; higher forces may damage the filters.		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Upstream tips
Do not centrifuge Filter Cartridges at relative centrifugal forces greater than 10,000 x g; higher forces may damage the filters.
Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA was then extracted using a specific kit and according to the manufacturer’s instructions (RecoverAll™ Total Nucleic Acid Isolation – cat 1975, AMBION®). The DNA concentration was measured at 260 nm. DNA quality was determined by electrophoresis on a 1% agarose gel running with 0.5X TBE buffer at room temperature for 30 min at 100V. Each sample was compared to a ladder (Smartladder®, Eurogentec) and classified according to their smear as “highly degraded” if only small DNA fragments were present, or “slightly degraded” if large DNA fragments were found (Figure 1).

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Manufacturer protocol

Download the product protocol from Fisher Scientific for Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE below.

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