RNeasy Plant Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Both enzymatic and mechanical lysis can be used for yeast. Enzymatic lysis doesn't require additional lab equipment, but takes longer than mechanical disruption.

Protocol tips
Both enzymatic and mechanical lysis can be used for yeast. Enzymatic lysis doesn't require additional lab equipment, but takes longer than mechanical disruption.

Publication protocol

Total RNA extraction from frozen mycelium was carried out using the RNeasy Plant Mini kit (QIAGEN) according to the manufacturer’s instructions for isolation of total RNA from filamentous fungi. RNA concentration and purity were determined using a NanoDrop ND-1000 (NanoDrop Technologies) and integrity of RNA was analyzed using an Agilent 2100 Bioanalyzer (Agilent Technologies).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plant Mini Kit below.

Download manufacturer protocol

Videos

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