E.Z.N.A.® Fungal RNA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Cell disruption was performed in a cell homogenizer (Retsch MM200) with glass beads (Qiagen) at 30 hits s−1 for 3 min.	If RNA remains on the column, heat DEPC water to 65 °C prior to elution	The isolated RNA was quantified and treated with TURBO DNA‐free kit (Ambion)

Upstream tips
Cell disruption was performed in a cell homogenizer (Retsch MM200) with glass beads (Qiagen) at 30 hits s−1 for 3 min.
Protocol tips
If RNA remains on the column, heat DEPC water to 65 °C prior to elution
Downstream tips
The isolated RNA was quantified and treated with TURBO DNA‐free kit (Ambion)

Publication protocol

RNA was isolated with the Fungal RNA Extraction kit from Omega according to the RB protocol with minor changes of cell disruption. Cell disruption was performed in a cell homogenizer (Retsch MM200) with glass beads (Qiagen) at 30 hits s−1 for 3 min. The isolated RNA was quantified and treated with TURBO DNA‐free kit (Ambion). RNA samples were diluted to a final concentration of 50 ng μl−1 in DEPC water.

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Manufacturer protocol

Download the product protocol from Omega Bio Tek for E.Z.N.A.® Fungal RNA Kit below.

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