RNeasy Plant Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Both enzymatic and mechanical lysis can be used for yeast. Enzymatic lysis doesn't require additional lab equipment, but takes longer than mechanical disruption.

Protocol tips
Both enzymatic and mechanical lysis can be used for yeast. Enzymatic lysis doesn't require additional lab equipment, but takes longer than mechanical disruption.

Publication protocol

For RNA isolation strains were grown vegetatively or asexually. Mycelia was harvested through sterile filters (Miracloth) and immediately frozen in liquid nitrogen. Frozen mycelia were ground with a table mill (Retsch) directly before RNA extraction. RNA was extracted with the RNeasy Plant Miniprep Kit (Qiagen) according to manufacturer’s instructions. cDNA was transcribed from 0.8 μg RNA with the QuantiTect Reverse Transcription Kit (Qiagen).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plant Mini Kit below.

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