PolyATtract® mRNA Isolation Systems

Protocol tips

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	To confirm the nbs1 gene structure and sequence as predicted by the Broad Institute genome sequence, reverse-transcription (RT) PCR was used on purified poly-A RNA from a J6;5x4 K+6 mushroom (SuperScript One-Step RT-PCR for Long Templates, Invitrogen; PolyATtract mRNA Isolation System III, Promega)

Protocol tips
To confirm the nbs1 gene structure and sequence as predicted by the Broad Institute genome sequence, reverse-transcription (RT) PCR was used on purified poly-A RNA from a J6;5x4 K+6 mushroom (SuperScript One-Step RT-PCR for Long Templates, Invitrogen; PolyATtract mRNA Isolation System III, Promega)

Publication protocol

To confirm the nbs1 gene structure and sequence as predicted by the Broad Institute genome sequence, reverse-transcription (RT) PCR was used on purified poly-A RNA from a J6;5x4 K+6 mushroom (SuperScript One-Step RT-PCR for Long Templates, Invitrogen; PolyATtract mRNA Isolation System III, Promega)

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Manufacturer protocol

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