TRI Reagent® MRC

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	Total RNA was extracted by TRI® reagent (Molecular Research Center, Inc) and Poly(A)+ mRNA was furthered isolated using the PolyATract® mRNA isolation system (Promega).

Protocol tips
Total RNA was extracted by TRI® reagent (Molecular Research Center, Inc) and Poly(A)+ mRNA was furthered isolated using the PolyATract® mRNA isolation system (Promega).

Publication protocol

Total RNA was extracted by TRI® reagent (Molecular Research Center, Inc) and Poly(A)+ mRNA was furthered isolated using the PolyATract® mRNA isolation system (Promega). First strand cDNA was synthesized using SuperScript™ III First-Strand Synthesis System for qRT-PCR (Invitrogen). Two different template switching oligos were employed for each developmental stage. Reverse transcription was performed at 42°C for 90 minutes. Second strand cDNA was then synthesized by low cycle primer extension using Advantage® 2 polymerase (Clontech). Double-stranded cDNA was purified by QIAquick PCR purification kit (Qiagen) and checked by electrophoresis on a 1.5% agarose gel.

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Manufacturer protocol

Download the product protocol from Molecular Research Center, Inc. for TRI Reagent® MRC below.

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