TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was isolated using TRIzol (Invitrogen) and resolved alongside a single-stranded RNA ladder (New England Biolabs) in a 6% polyacrylamide gel containing 7 M urea. The RNA was transferred to a Hybond-XL nylon membrane (Amersham) and crosslinked by UV irradiation (120 mJ; UV Stratalinker 2400, Stratagene). Crosslinked membranes were blocked with ULTRAhyb-Oligo solution (Ambion) for 30 min at 42°C, then incubated overnight at 47°C with DNA probes complementary to the SRP RNA (Supplementary Table S3), which had been 5′ end radiolabeled according to manufacturer's instructions (KinaseMax, Ambion). Hybridized membranes were washed four times, 30 min each (2x SSC, 0.5% SDS), then imaged using a storage phosphor screen (Amersham).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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