PureLink™ RNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For lysis of yeast, extra enzymatic or mechanical disruption is recommended.	Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required	DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit

Upstream tips
For lysis of yeast, extra enzymatic or mechanical disruption is recommended.
Protocol tips
Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required
Downstream tips
DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit

Publication protocol

Total RNA was purified using the purelink RNA purification kit (Invitrogen) and was used as the template for the first strand cDNA synthesis using the Superscript III cDNA synthesis kit (Invitrogen). Relative expression level of selected genes was measured by real time PCR using power SYBR qPCR premix reagents (Invitrogen) in a Realplex system (Eppendorf). Primer efficiency was determined by serially diluting the cDNA and monitoring DNA amplification by real-time PCR. Primers for qPCR used in this study are listed in Table S3. Gene-expression levels were normalized using the endogenous control gene TEF1.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ RNA Mini Kit below.

Download manufacturer protocol

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