PureLink Genomic DNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

RNA Isolation, gDNA Isolation, and PCR.
Total RNA was isolated using TRIzol (Invitrogen) and was DNase-treated (Invitrogen). cDNA synthesis was performed by using random hexamers and SuperScript II (Invitrogen). Primers are listed in Table S2. gDNA was used for bisulfite and restriction PCR following isolation using the PureLink Genomic DNA Mini Kit (Invitrogen). Bisulfite PCR was performed on bisulfite-converted gDNA (EZ DNA Methylation Direct Kit; Zymo) (7, 28). See SI Materials and Methods.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink Genomic DNA Mini Kit below.

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