PureLink Genomic DNA Mini Kit

DNA isolation / purification Tissue - eye

Experiment
DNA isolation / purification Tissue - eye
Product
PureLink Genomic DNA Mini Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

RNA Isolation, gDNA Isolation, and PCR.
Total RNA was isolated using TRIzol (Invitrogen) and was DNase-treated (Invitrogen). cDNA synthesis was performed by using random hexamers and SuperScript II (Invitrogen). Primers are listed in Table S2. gDNA was used for bisulfite and restriction PCR following isolation using the PureLink Genomic DNA Mini Kit (Invitrogen). Bisulfite PCR was performed on bisulfite-converted gDNA (EZ DNA Methylation Direct Kit; Zymo) (7, 28). See SI Materials and Methods.

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Reviews

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Discussion

Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - eye using PureLink Genomic DNA Mini Kit from Thermo Fisher Scientific.

Paper title
Analysis of DNA methylation reveals a partial reprogramming of the Müller glia genome during retina regeneration
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink Genomic DNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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