TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.	RNA was purified using a Qiagen RNeasy kit, and treated with Ambion Turbo DNase

Protocol tips
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.
Downstream tips
RNA was purified using a Qiagen RNeasy kit, and treated with Ambion Turbo DNase

Publication protocol

RNA was extracted using the TRIzol reagent (Invitrogen), purified using a Qiagen RNeasy kit, and treated with Ambion Turbo DNase. RNA quality was assessed using gel electrophoresis and a Nanodrop (Thermo Scientific). Q RT-PCR was performed using a Qiagen QuantiTect SYBR Green RT-PCR kit and an ABI 7300 machine, according to the manufacturer’s instructions. Reactions were performed in triplicate, and actin was used as the endogenous control. Data were analyzed using the ABI 7300 software.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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