RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	"- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C"

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
"- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C"

Publication protocol

Samples taken in the exponential growth phase were employed for RNA isolation using the RNeasy Mini Kit (Qiagen, Hilden, Germany), with both an on- and off-column gDNA digestion step. RNA quantity and the absence of gDNA and other contaminants was assayed with the DropSense16 system (Trinean, Ghent, Belgium).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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