TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

P. pastoris GS115 was grown in BMDY, BMRY, or BMDRY to OD600 1.2–1.4, and then cells were harvested by centrifugation (12,000 g × 5 min) at 4 °C and immediately stored at −80 °C. Total RNA from P. pastoris cells was extracted with Trizol reagent (Invitrogen) followed by digestion with DNase I (RNase-free; MBI Fermentas, St. Leon-Roth, Germany) to remove trace DNA. cDNA was synthesized from each RNA sample (1 μg) using the RevertAid H Minus First Strand cDNA Synthesis Kit (K1631; MBI Fermentas). Real-time PCR assays were performed in the IQ5 Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA) using the SuperReal PreMix SYBR Green I Kit (Toyobo, Osaka, Japan)

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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