RNeasy Plant Mini Kit

Protocol tips

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	RNA of seven days old, solid cultures of the strains 12–43, E6 and ∆dhc2 was isolated using RNeasy Plant Mini Kit performing an additional DNAse digestion with RNase-Free DNase Set (both Qiagen, Hilden,Germany). RNA-sequencing was performed by LGC Genomics, Berlin (Germany) with mRNA-based cDNA-libraries constructed from sequencing adaptors ligated to cDNA fragments

Protocol tips
RNA of seven days old, solid cultures of the strains 12–43, E6 and ∆dhc2 was isolated using RNeasy Plant Mini Kit performing an additional DNAse digestion with RNase-Free DNase Set (both Qiagen, Hilden,Germany). RNA-sequencing was performed by LGC Genomics, Berlin (Germany) with mRNA-based cDNA-libraries constructed from sequencing adaptors ligated to cDNA fragments

Publication protocol

RNA of seven days old, solid cultures of the strains 12–43, E6 and ∆dhc2 was isolated using RNeasy Plant Mini Kit performing an additional DNAse digestion with RNase-Free DNase Set (both Qiagen, Hilden,Germany). RNA-sequencing was performed by LGC Genomics, Berlin (Germany) with mRNA-based cDNA-libraries constructed from sequencing adaptors ligated to cDNA fragments

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Manufacturer protocol

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