TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Protocol tips
Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

RNA was extracted using TriZol (Life technologies, Carlsbad, USA) and purified using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). Quality of RNA was checked using the BioAnalyzer and sent to ServiceXS (Leiden, the Netherlands) for Illumina Next Generation Sequencing. RNAseq data have been deposited at NCBI under BioProject PRJNA323434.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Yeast - Schizophyllum commune using TRIzol Reagent from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Yeast - Schizophyllum commune using TRIzol Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.