TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNAs were prepared from HeLa cells or yeast cells using the TRIzol reagent (Invitrogen Life Technologies). To obtain 5S, 5.8S or 18S rRNA, total RNAs were applied to a reversed-phase LC system (LC-20A, Shimadzu) equipped with a capillary PLRP-S 4000 column (2-mm i.d. × 100 mm in length, 8-μm particle size; Polymer Laboratories), eluted with a 60-min linear gradient from 11.6% to 14% acetonitrile in 100 mM triethylammonium acetate (pH 7.0) including 0.1 mM ammonium phosphate dibasic at a flow rate of 50 μl/min at 60°C and fractionated by monitoring ultraviolet absorption at 260 nm (33). Only rRNAs with >95% purity were used.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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