TRIzol Reagent

RNA isolation / purification Yeast - Schizosaccharomyces pombe

Experiment
RNA isolation / purification Yeast - Schizosaccharomyces pombe
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNAs were prepared from HeLa cells or yeast cells using the TRIzol reagent (Invitrogen Life Technologies). To obtain 5S, 5.8S or 18S rRNA, total RNAs were applied to a reversed-phase LC system (LC-20A, Shimadzu) equipped with a capillary PLRP-S 4000 column (2-mm i.d. × 100 mm in length, 8-μm particle size; Polymer Laboratories), eluted with a 60-min linear gradient from 11.6% to 14% acetonitrile in 100 mM triethylammonium acetate (pH 7.0) including 0.1 mM ammonium phosphate dibasic at a flow rate of 50 μl/min at 60°C and fractionated by monitoring ultraviolet absorption at 260 nm (33). Only rRNAs with >95% purity were used.

Full paper   Login or join for free to view the full paper.

Reviews

TRIzol Reagent from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Yeast - Schizosaccharomyces pombe using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
A mass spectrometry-based method for comprehensive quantitative determination of post-transcriptional RNA modifications: the complete chemical structure of ribosomal RNAs
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Yeast - Schizosaccharomyces pombe using TRIzol Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms