E.Z.N.A.® Yeast RNA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The following might help to increase RNA yield - Heat the DEPC Water to 70°C before adding to the column. - Increase the incubation time to 5 minutes. - Increase the elution volume. - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration). - Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume).	Isolated RNA was treated with RNase-free DNase I

Protocol tips
The following might help to increase RNA yield - Heat the DEPC Water to 70°C before adding to the column. - Increase the incubation time to 5 minutes. - Increase the elution volume. - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration). - Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume).
Downstream tips
Isolated RNA was treated with RNase-free DNase I

Publication protocol

RNA was isolated using an E.Z.N.A.® Yeast RNA Kit (OMEGA). Isolated RNA was treated with RNase-free DNase I (Fermentas) to remove contaminating genomic DNA. RNAs were reverse-transcribed using the iScript cDNA Synthesis Kit (Bio-Rad). Quantitative Real-Time RT-PCR (qRT-PCR) was performed using SYBR Select Master Mix (Life Technologies) with each primer sets (Supplementary Table S2). All reactions were performed in triplicate. Data analysis was performed by StepOneTM software. The threshold cycle number (CT) values were normalized by subtracting the mean CT value of histone H2B (htb1) mRNA. The relative levels of expression of mitochondrial-encoded RNAs (mt-RNAs) were calculated by using the 2−ΔΔCt method.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Yeast - Schizosaccharomyces pombe using E.Z.N.A.® Yeast RNA Kit from Omega Bio Tek.

Manufacturer protocol

Download the product protocol from Omega Bio Tek for E.Z.N.A.® Yeast RNA Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Yeast - Schizosaccharomyces pombe using E.Z.N.A.® Yeast RNA Kit from Omega Bio Tek. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.