AllPrep DNA/RNA Mini Kit

DNA isolation / purification Tissue - eye

Experiment

DNA isolation / purification Tissue - eye

Product

AllPrep DNA/RNA Mini Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

3.6.2. Isolate DNA Using the AllPrep DNA/RNA Micro Kit
Microdissect the desired cells into 28 μl of Buffer RLT Plus. The cells can be stored in the buffer at −80°C until you are ready to isolate the RNA and DNA. Multiple dissections can be combined prior to isolation of nucleic acid up to 350 μl.

Follow the steps as listed for “Simultaneous Purification of Genomic DNA and Total RNA from Microdissected Cryosections” in the manufacturer's instructions. Elute the DNA with 20 μl of Buffer EB (preheated to 70°C).

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Reviews

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Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - eye using AllPrep DNA/RNA Mini Kit from Qiagen.

Paper title

Use of Laser Capture Microdissection for Analysis of Retinal mRNA/miRNA Expression and DNA Methylation

Full paper

Manufacturer protocol

Download the product protocol from Qiagen for AllPrep DNA/RNA Mini Kit below.

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Videos

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