TRI Reagent® Sigma

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	total DNA was liberated from cells by heating their suspension in water for 5 minutes at 95°C and removing cellular debris by spinning at 13200×g for 3 minutes at room temperature. For total RNA preparation, cells were pulverized in liquid nitrogen and RNA was isolated using TRI Reagent (Sigma) followed by purification with NucleoSpin RNA Clean-up (Macherey-Nagel). For total cDNA preparation, 2 µg of total RNA was used with RevertAid M-MuLV Reverse Transcriptase (Fermentas) according to the manufacturer’s recommended protocol.

Protocol tips

total DNA was liberated from cells by heating their suspension in water for 5 minutes at 95°C and removing cellular debris by spinning at 13200×g for 3 minutes at room temperature. For total RNA preparation, cells were pulverized in liquid nitrogen and RNA was isolated using TRI Reagent (Sigma) followed by purification with NucleoSpin RNA Clean-up (Macherey-Nagel). For total cDNA preparation, 2 µg of total RNA was used with RevertAid M-MuLV Reverse Transcriptase (Fermentas) according to the manufacturer’s recommended protocol.

Publication protocol

total DNA was liberated from cells by heating their suspension in water for 5 minutes at 95°C and removing cellular debris by spinning at 13200×g for 3 minutes at room temperature. For total RNA preparation, cells were pulverized in liquid nitrogen and RNA was isolated using TRI Reagent (Sigma) followed by purification with NucleoSpin RNA Clean-up (Macherey-Nagel). For total cDNA preparation, 2 µg of total RNA was used with RevertAid M-MuLV Reverse Transcriptase (Fermentas) according to the manufacturer’s recommended protocol.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Yeast - Schizosaccharomyces pombe using TRI Reagent® Sigma from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Yeast - Schizosaccharomyces pombe using TRI Reagent® Sigma from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.