TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA from Ustilago cells (three experiments in triplicates) or Arabidopsis plantlets (four experiments with 150 plants each) was isolated using Trizol (Invitrogen) and purified with QIAGEN columns (Cat. 28104). RNA concentration was measured by its absorbance at 260 nm and its integrity observed by electrophoresis in denaturing agarose gels. cDNA synthesis and labeling and hybridization of the microarrays were performed by Roche NimbleGen Inc.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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