|Aliquot rDNase and store at -20 °C.|
|Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.|
NucleoSpin® RNA from Macherey Nagel has not yet been reviewed for this experiment
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4 years ago
Author: Paul G. Macon
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
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