Direct-zol™ RNA MiniPrep Plus

RNA isolation / purification Bacteria - Gram negative Borrelia burgdorferi

Experiment

RNA isolation / purification Bacteria - Gram negative Borrelia burgdorferi

Product

Direct-zol™ RNA MiniPrep Plus from Zymo Research

Manufacturer

Zymo Research

Buy product Write review

Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Tough-to-lyse samples (i.e.,gram-positive bacteria, yeast, fungi, animal tissue, plant, etc.) can be homogenized in TRI Reagent® (supplied with kit) with a mortar/pestle, dounce, tissue grinder, or bead beating with a high-speed homogenizer prior to RNA Purification.

Upstream tips
Tough-to-lyse samples (i.e.,gram-positive bacteria, yeast, fungi, animal tissue, plant, etc.) can be homogenized in TRI Reagent® (supplied with kit) with a mortar/pestle, dounce, tissue grinder, or bead beating with a high-speed homogenizer prior to RNA Purification.

Publication protocol

RNA was isolated from 500 μl of cell suspension using the Zymo RNA Direct-Zol miniprep kit (Zymo). Isolated RNA was eluted in 35 μl RNase-free water and stored at -80C. RNA quantity and integrity was assayed by microfluidic analysis using an Agilent 2100 Bioanalyzer and with the Agilent RNA 6000 Nano chip kit (Agilent) and only samples with a RIN score > 9 were used for library construction.

Full paper Login or join for free to view the full paper.

Reviews

Direct-zol™ RNA MiniPrep Plus from Zymo Research has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Comment View 4 comments

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Borrelia burgdorferi using Direct-zol™ RNA MiniPrep Plus from Zymo Research.

Paper title

RNA-Seq of in Multiple Phases of Growth Reveals Insights into the Dynamics of Gene Expression, Transcriptome Architecture, and Noncoding RNAs

Full paper

Manufacturer protocol

Download the product protocol from Zymo Research for Direct-zol™ RNA MiniPrep Plus below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Bacteria - Gram negative Borrelia burgdorferi using Direct-zol™ RNA MiniPrep Plus from Zymo Research. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

Outsource experiment