RNeasy Plus Mini Kit

RNA isolation / purification Bacteria - Gram negative Chlamydia pneumoniae

Experiment
RNA isolation / purification Bacteria - Gram negative Chlamydia pneumoniae
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60C.

Use water to elute the RNA that is warmed to ~60C.

Publication protocol

Eukaryotic and bacterial RNA was isolated using an RNeasy Plus RNA minikit (Qiagen) according to the manufacturer's instructions.

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Reviews

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Chlamydia pneumoniae using RNeasy Plus Mini Kit from Qiagen.

Paper title
AP-1 Transcription Factor Serves as a Molecular Switch between Replication and Persistence
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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