|Store DNase I at -20°C immediately upon receipt. Do not store DNase I at room temperature.|
|Ensure that the sample is fully homogenized before proceeding to 60°C incubation.
After adding Elution Buffer (E7) to the sample, pipet up and down to resuspend the magnetic beads before incubation.
ChargeSwitch™ Total RNA Cell Kit from Thermo Fisher Scientific has not yet been reviewed for this experiment
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2 years ago
Author: Paul G. Macon
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
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