SV Total RNA Isolation System

RNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Experiment

RNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Product

SV Total RNA Isolation System from Promega

Manufacturer

Promega

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not freeze-thaw the DNase more than three times after rehydration. After adding ethanol to the RNA wash solution, label the bottle so that you fellow researchers don't add any extra.	For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Protocol tips
Do not freeze-thaw the DNase more than three times after rehydration. After adding ethanol to the RNA wash solution, label the bottle so that you fellow researchers don't add any extra.
Downstream tips
For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Publication protocol

Total RNA of transfected cells was extracted with SV Total RNA Isolation System (Promega, USA) according to the manufacturer's instructions.

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Reviews

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Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Helicobacter pylori using SV Total RNA Isolation System from Promega.

Paper title

Novel effects of Helicobacter pylori CagA on key genes of gastric cancer signal transduction: a comparative transfection study.

Full paper

Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

Download manufacturer protocol

Videos

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