RNeasy Protect Bacteria Mini Kit

RNA isolation / purification Bacteria - Gram negative Salmonella typhi

Experiment

RNA isolation / purification Bacteria - Gram negative Salmonella typhi

Product

RNeasy Protect Bacteria Mini Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
To ensure reliable and reproducable gene expression, minimal media is recommended, though complex media also work with this kit.		RNA protect only stabilizes the RNA and lyses the bacteria, for further purification RNeasy mini or midi kits are recommended by the manufacturer.

Upstream tips
To ensure reliable and reproducable gene expression, minimal media is recommended, though complex media also work with this kit.
Downstream tips
RNA protect only stabilizes the RNA and lyses the bacteria, for further purification RNeasy mini or midi kits are recommended by the manufacturer.

Publication protocol

RNA extraction from bacterial cells was performed with RNeasy Protect Bacterial Mini Kit (Qiagen, India) following the manufacturer’s instructions for Gram-negative bacteria.

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Reviews

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Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Salmonella typhi using RNeasy Protect Bacteria Mini Kit from Qiagen.

Paper title

grows vigorously on seafood and expresses its virulence and stress genes at different temperature exposure

Full paper

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Protect Bacteria Mini Kit below.

Download manufacturer protocol

Videos

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