TRIzol™ Max™ Bacterial RNA Isolation Kit
RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus
Protocol tips
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| This product stabilizes bacterial RNA and aids in bacterial lysis, TRIzol or a similar product is required for further processing. |
Publication protocol
RNA was isolated from three parallel independent cultures for both conditions. One and a half ml cultures were harvested in exponential phase at an OD600 of 1.0 and rinsed once with PBS. Total RNA was isolated from the pellet with Trizol Max Bacterial (ThermoFisher Scientific) according to the manufacturer’s protocol. The quantity of total RNA was determined by Nanodrop 2000 (Thermo Scientific, Wilmington, DE, USA) and QuantiFluor-ST Fluorometer-Ribogreen (Promega, USA), and quality and integrity of the RNA was determined by electrophoresis in the Bioanalyzer 2100 (Agilent Technologies, USA). Yield of RNA per optical density was 0.6-times lower in condition E than condition I. RNA integrity (RIN) was higher than 7.8 in all RNA samples. No differences in the electrophoretic patterns of the RNA in the two conditions were evident.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Paul G. Macon
Some help with RNA isolation using Trizol
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
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Manufacturer protocol
Download the product protocol from Thermo Fisher Scientific for TRIzol™ Max™ Bacterial RNA Isolation Kit below.
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