RNeasy Lipid Tissue Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. All centrifugation steps should be performed at 15–25°C except for phase separation

Protocol tips
If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. All centrifugation steps should be performed at 15–25°C except for phase separation

Publication protocol

The RNA was purified using a Qiagen RNeasy Prep kit according to the RNeasy lipid tissue directions. Briefly, samples were thawed on ice and then pelleted at 3,200 x g for 10 min. The supernatants were removed and the pellets dried for 10 min on ice. Each pellet was resuspended in 1 ml of Trizol and transferred to a bead beater tube containing Lysing Matrix B (MP). Cells were lysed on a FastPrep-24 bead beater for 45 s at 6 m/s. Samples were cooled on ice and then mixed with 200 μl chloroform. Samples were pelleted at 16,100 xg for 15 min at 4°C. The upper aqueous layer was transferred to a new tube and then RNA was isolated according to the Qiagen RNeasy lipid tissue directions and eluted twice, each time in 50 μl of water.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Lipid Tissue Mini Kit below.

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