RiboPure™ RNA Purification Kit, bacteria

RNA isolation / purification Bacteria - Gram positive Clostridium difficile

Experiment

RNA isolation / purification Bacteria - Gram positive Clostridium difficile

Product

RiboPure™ RNA Purification Kit, bacteria from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
This kit is suitable for the cell samples which were difficult to disrupt.		When checking the yield by means of a specrtophotometer, use the supplied TE buffer to zero the machine

Upstream tips
This kit is suitable for the cell samples which were difficult to disrupt.
Downstream tips
When checking the yield by means of a specrtophotometer, use the supplied TE buffer to zero the machine

Publication protocol

RNA was isolated from collected bacterial pellets using the RiboPure-Bacteria kit (Ambion) according to the manufacturer’s recommendations.

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Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram positive Clostridium difficile using RiboPure™ RNA Purification Kit, bacteria from Thermo Fisher Scientific.

Paper title

Effects of ciprofloxacin on the expression and production of exotoxins by

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RiboPure™ RNA Purification Kit, bacteria below.

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Videos

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