E.Z.N.A.® Total RNA Kit I

RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis

Experiment

RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis

Product

E.Z.N.A.® Total RNA Kit I from Omega Bio Tek

Manufacturer

Omega Bio Tek

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	"The following might help to increase RNA yield - Heat the DEPC Water to 70°C before adding to the column. - Increase the incubation time to 5 minutes. - Increase the elution volume. - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration). - Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume)."

Protocol tips
"The following might help to increase RNA yield - Heat the DEPC Water to 70°C before adding to the column. - Increase the incubation time to 5 minutes. - Increase the elution volume. - Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration). - Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume)."

Protocol tips

"The following might help to increase RNA yield
- Heat the DEPC Water to 70°C before adding to the column.
- Increase the incubation time to 5 minutes.
- Increase the elution volume.
- Repeat the elution step with fresh DEPC Water (this may increase the yield, but decrease the concentration).
- Repeat the elution step using the eluate from the first elution (this may increase yield while maintaining elution volume)."

Publication protocol

this protocol uses both chemical (phenol; AppliChem, Darmstadt, Germany) and mechanical (glass beads; Sigma-Aldrich, St. Louis, MO) lysis together with column systems for RNA isolation (E.Z.N.A Total RNA kit I, Omega Bio-Tek, Norcross, GA). Genomic DNA was digested with DNase I (Thermo Scientific) following the manufacturer's instructions

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Reviews

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Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis using E.Z.N.A.® Total RNA Kit I from Omega Bio Tek.

Paper title

Assessing and reducing sources of gene expression variability in Staphylococcus epidermidis biofilms.

Full paper

Manufacturer protocol

Download the product protocol from Omega Bio Tek for E.Z.N.A.® Total RNA Kit I below.

Download manufacturer protocol

Videos

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