Direct-zol RNA Kits

RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes

Experiment

RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes

Product

Direct-zol RNA Kits from Zymo Research

Manufacturer

Zymo Research

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Tough-to-lyse samples (i.e.,gram-positive bacteria, yeast, fungi, animal tissue, plant, etc.) can be homogenized in TRI Reagent® (supplied with kit) with a mortar/pestle, dounce, tissue grinder, or bead beating with a high-speed homogenizer prior to RNA Purification.

Upstream tips
Tough-to-lyse samples (i.e.,gram-positive bacteria, yeast, fungi, animal tissue, plant, etc.) can be homogenized in TRI Reagent® (supplied with kit) with a mortar/pestle, dounce, tissue grinder, or bead beating with a high-speed homogenizer prior to RNA Purification.

Publication protocol

Total RNA in TRIzol was isolated with Direct-zol RNA miniprep (Zymo research) according to the manufacturer’s recommendations

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Reviews

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Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes using Direct-zol RNA Kits from Zymo Research.

Paper title

Paediatric obstructive sleep apnoea syndrome (OSAS) is associated with tonsil colonisation by

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Manufacturer protocol

Download the product protocol from Zymo Research for Direct-zol RNA Kits below.

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Videos

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