RNeasy Plus Mini Kit

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	Quantitative Real-time PCR (qRT-PCR). RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) and converted into 1 µg of cDNA (Thermo Scientific).

Protocol tips
Quantitative Real-time PCR (qRT-PCR). RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) and converted into 1 µg of cDNA (Thermo Scientific).

Publication protocol

Quantitative Real-time PCR (qRT-PCR). RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) and converted into 1 µg of cDNA (Thermo Scientific). qRT-PCR was performed as previously described9 using gene-specific primers (Supplementary Table S2). Gene expression levels relative to GAPDH were determined with the formula An external file that holds a picture, illustration, etc.
Object name is mt2015226e1.jpg. Fold increase in gene-expression compared to controls was calculated with the formula An external file that holds a picture, illustration, etc.
Object name is mt2015226e2.jpg. Samples for which no amplification could be detected were assigned a Ct value of 40, resembling the total number of PCR cycles.



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Manufacturer protocol

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