GenElute™ Mammalian Total RNA Miniprep Kit

RNA isolation / purification Cells - primary human pancreatic stellate cells

Experiment
RNA isolation / purification Cells - primary human pancreatic stellate cells
Product
GenElute™ Mammalian Total RNA Miniprep Kit from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
Thereafter, cells were activated with TGF-β1 (5 ng/ml) for 24 h, then total RNA was isolated using the GenElute™ Mammalian Total RNA Miniprep Kit and the RNA amount was measured by a NanoDrop® ND-1000 Spectrophotometer (Wilmington, DE). Subsequently, cDNA was synthesized with iScript™ cDNA Synthesis Kit (BioRad, Veenendaal, The Netherlands). 10ng cDNA was used for each PCR reaction.

Publication protocol

hPSCs were seeded in 24 well plate (2 × 104 cells/well) or 12 well plate (6 × 104 cells/well) in complete medium. After 18 h, cells were transfected with anti-miR-199a and anti-miR-214 hairpin inhibitors for 24 h. Thereafter, cells were activated with TGF-β1 (5 ng/ml) for 24 h, then total RNA was isolated using the GenElute™ Mammalian Total RNA Miniprep Kit and the RNA amount was measured by a NanoDrop® ND-1000 Spectrophotometer (Wilmington, DE). Subsequently, cDNA was synthesized with iScript™ cDNA Synthesis Kit (BioRad, Veenendaal, The Netherlands). 10ng cDNA was used for each PCR reaction.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human pancreatic stellate cells using GenElute™ Mammalian Total RNA Miniprep Kit from Sigma-Aldrich.

Paper title
MicroRNA-199a and -214 as potential therapeutic targets in pancreatic stellate cells in pancreatic tumor
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for GenElute™ Mammalian Total RNA Miniprep Kit below.

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