RNeasy Plus Micro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- When processing <2 µg tissue, carrier RNA may be added to the lysate. Foaming can be reduced by adding Reagent DX at a final concentration of 0.5% (v/v) before disruption and homogenization. - Add 4 volumes of ethanol (96–100%) to Buffer RPE for a working solution. before homogenization

Upstream tips
- When processing <2 µg tissue, carrier RNA may be added to the lysate. Foaming can be reduced by adding Reagent DX at a final concentration of 0.5% (v/v) before disruption and homogenization. - Add 4 volumes of ethanol (96–100%) to Buffer RPE for a working solution. before homogenization

Publication protocol

Besides mRNA extraction from the FACS and LMD groups, 10-μm-thick whole liver tissue slides, sectioned from frozen tissue, were homogenised and lysed in RTL plus buffer with 1% 2-mercaptoethanol. Total mRNA from whole liver, FACS and microdissection samples was extracted using the RNeasy Plus Micro Kit (Qiagen) according to the manufacturer’s instructions.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Liver using RNeasy Plus Micro Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Micro Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Liver using RNeasy Plus Micro Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.