SV Total RNA Isolation System
RNA isolation / purification Tissue - Human Liver
Publication protocol
Hepatic tissue samples were collected using RNAlater ® (Thermo Fisher Scientific, Inc., CA, USA) and stored at -70oC until ribonucleic acid (RNA) extraction. RNA from hepatic tissue was isolated using the spin (SV) Total RNA Isolation System kit (Promega Corporation, Madison, WI, USA), according to the manufacturer’s specifications.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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