PureLink™ FFPE RNA Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To increase RNA concentration, reduce elution volume. - Performing a second elution step increases the RNA yield by up to 30%. - Pre-warming the elution buffer to 65°C increases the RNA yield by up to 15-20%	- The purified RNA is suitable for downstream applications such as reverse transcription, RT-PCR, and real-time quantitative RT-PCR (qRT-PCR).

Protocol tips
- To increase RNA concentration, reduce elution volume. - Performing a second elution step increases the RNA yield by up to 30%. - Pre-warming the elution buffer to 65°C increases the RNA yield by up to 15-20%
Downstream tips
- The purified RNA is suitable for downstream applications such as reverse transcription, RT-PCR, and real-time quantitative RT-PCR (qRT-PCR).

Publication protocol

In a first step, seven RNA extraction kits (Table 1) were tested on rat renal tissue. 12 samples were analyzed for each kit: Four samples with one rat whole kidney section, four samples with two whole kidney sections and four samples with 80–101 LCM glomerular cross-sections. All sections had a thickness of 5 lm. Later, the four kits, which yielded the highest amount of RNA extracted from rat whole kidney sections were used to extract RNA from human renal tissue: Six samples were analyzed for each kit: Four samples with two human kidney biopsy sections and two samples with 80–160 LCM glomerular cross-sections. Human samples had a thickness of 10 lm. Each of the seven different kits was performed according to the manufacturer’s instructions under RNAse-free conditions. The volume of eluate was set to 25 lL in the case of the rat tissues and 15 lL in the case of the human tissue

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ FFPE RNA Isolation Kit below.

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