Absolutely RNA FFPE Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If RNA degradation is high, ensure that Proteinase K digestion is carried out at 55°C. - If RNA yield is poor ensure that the 90% sulfolane and the pre-filter spin cup filtrate were combined at a 1:1 ratio prior to loading the RNAbinding spin cup.	- If Final RNA concentration is too low for use in subsequent applications use a smaller volume of Elution Buffer, ensuring that the surface of the fiber matrix is completely covered.

Protocol tips
- If RNA degradation is high, ensure that Proteinase K digestion is carried out at 55°C. - If RNA yield is poor ensure that the 90% sulfolane and the pre-filter spin cup filtrate were combined at a 1:1 ratio prior to loading the RNAbinding spin cup.
Downstream tips
- If Final RNA concentration is too low for use in subsequent applications use a smaller volume of Elution Buffer, ensuring that the surface of the fiber matrix is completely covered.

Publication protocol

In a first step, seven RNA extraction kits (Table 1) were
tested on rat renal tissue. 12 samples were analyzed for each kit: Four samples with one rat whole kidney section, four samples with two whole kidney sections and four samples with 80–101 LCM glomerular cross-sections. All sections had a thickness of 5 lm.
Later, the four kits, which yielded the highest amount of RNA extracted from rat whole kidney sections were used to extract RNA from human renal tissue: Six samples were analyzed for each kit: Four samples with two human kidney biopsy sections and two samples with 80–160 LCM glomerular cross-sections. Human samples had a thickness of 10 lm. Each of the seven different kits was performed according to the manufacturer’s instructions under RNAse-free conditions. The volume of eluate was set to 25 lL in the case of the rat tissues and 15 lL in the case of the human tissue

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Kidney using Absolutely RNA FFPE Kit from Agilent Technologies.

Manufacturer protocol

Download the product protocol from Agilent Technologies for Absolutely RNA FFPE Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Kidney using Absolutely RNA FFPE Kit from Agilent Technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.