AllPrep DNA/RNA/miRNA Universal Kit

RNA isolation / purification Tissue - Human Lung

Experiment
RNA isolation / purification Tissue - Human Lung
Product
AllPrep DNA/RNA/miRNA Universal Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Whole lung tissue was homogenized prior to RNA extraction (BeadBlaster 24, Benchmark Scientific) in the presence of lysis buffer (RLT Plus, Qiagen). Total RNA from whole lung tissue and sorted alveolar macrophages and alveolar type II cells was extracted (AllPrep DNA/RNA Mini Kit, Qiagen) and quality and quantity were assessed (TapeStation 4200, Agilent).

Publication protocol

Whole lung tissue was homogenized prior to RNA extraction (BeadBlaster 24, Benchmark Scientific) in the presence of lysis buffer (RLT Plus, Qiagen). Total RNA from whole lung tissue and sorted alveolar macrophages and alveolar type II cells was extracted (AllPrep DNA/RNA Mini Kit, Qiagen) and quality and quantity were assessed (TapeStation 4200, Agilent).


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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep DNA/RNA/miRNA Universal Kit below.

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