RNAprep pure Kit
RNA isolation / purification Tissue - Human Placenta
Protocol tips
| Upstream tips |
|---|
| - This kit is designed for tissue samples of 20 mg |
| Protocol tips |
| - β-Mercaptoethanol (β-ME) must be added to Buffer RL before use. The final concentration of β-ME is 1% |
Publication protocol
According to the instructions, we extracted total RNA from the samples using TRIzol reagent (Invitrogen, Karlsruhe, Germany) and the RNAprep pure tissue kit (TIANGEN) (DP431) in a step-by-step manner. The integrity of the extracted RNA was tested via 1% agarose gel electrophoresis using the following criteria: there could be up to three bands; the ratio of 28 S rRNA/18 S rRNA should be 2; and the 5 S rRNA should not be too bright. The purity of the extracted RNA was measured by a UV spectrophotometer using the following criteria: the 260/280 nm absorbance ratio of the qualified sample should be between 1.8 and 2.1, with 2.0 being considered best. According to the concentration of each sample, we added 1000 ng to the 20 µl reverse transcription system and then examined each sample using reverse transcription with random primers following the recommendations of the Thermo Scientific RevertAid First Strand cDNA Synthesis Kit.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Tiangen for RNAprep pure Kit below.
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