Upstream tips |
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- This kit is designed for tissue samples of 20 mg |
Protocol tips |
- β-Mercaptoethanol (β-ME) must be added to Buffer RL before use. The final concentration of β-ME is 1% |
RNAprep pure Kit from Tiangen has not yet been reviewed for this experiment
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Discussion
4 years ago
Author: Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author: Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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