AllPrep DNA/RNA/miRNA Universal Kit

RNA isolation / purification Tissue - Mouse Adipose

Experiment

RNA isolation / purification Tissue - Mouse Adipose

Product

AllPrep DNA/RNA/miRNA Universal Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit purifies RNA from a maximum amount of 30 mg fresh or frozen tissue or 20 mg of tissue that has been stabilized with RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent can generally be processed.	- Perform all centrifugation steps at 20–25ºC in astandard microcentrifuge	- For low A260/A280 value use 10 mM Tris·Cl,* pH 7.5, not RNase-free water, to dilute the sample before measuring purity.

Upstream tips
- This kit purifies RNA from a maximum amount of 30 mg fresh or frozen tissue or 20 mg of tissue that has been stabilized with RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent can generally be processed.
Protocol tips
- Perform all centrifugation steps at 20–25ºC in astandard microcentrifuge
Downstream tips
- For low A260/A280 value use 10 mM Tris·Cl,* pH 7.5, not RNase-free water, to dilute the sample before measuring purity.

Publication protocol

Total RNA was prepared from isolated whole gonadal adipose tissues using the AllPrep DNA/RNA mini kit (Qiagen, Hilden, Germany).

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Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Adipose using AllPrep DNA/RNA/miRNA Universal Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep DNA/RNA/miRNA Universal Kit below.

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Videos

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