TRI Reagent® MRC

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Protocol tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Total RNA was extracted using the TRI Reagent® (Molecular Research Center; Part #: TR118) method, and isolated RNA was quantified using chip-based capillary electrophoresis (Agilent 2100 Bioanalyzer Pico Chip). RNA was digested with RNase free DNase I (Epicentre; Part # D9905K) and purified through minElute columns (Qiagen; Part #: 74204). Final RNA samples were quantified by O.D. measurement and re-quantified using chip-based capillary electrophoresis.

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Manufacturer protocol

Download the product protocol from Molecular Research Center, Inc. for TRI Reagent® MRC below.

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