RNeasy Lipid Tissue Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - All centrifugation steps should be performed at 15–25°C except for phase separation

Protocol tips
- If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - All centrifugation steps should be performed at 15–25°C except for phase separation

Publication protocol

Total RNA (tRNA) was extracted from approximately 100 mg of adipose tissue, using the RNeasy lipid kit (Qiagen Benelux, Venlo, Netherlands), with on-column DNAse treatment using RNAse-free DNAse (Qiagen Benelux, Venlo, Netherlands), according to the manufacturer’s protocol. tRNA was measured on a Nanodrop 1000 (Thermo Fisher Scientific, Waltham, MA, USA) and diluted to equal concentrations.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Lipid Tissue Mini Kit below.

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