SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not use DEPC-treated water, only water provided in the kit or nuclease-free water

Protocol tips
Do not use DEPC-treated water, only water provided in the kit or nuclease-free water

Publication protocol

To analyze gene expression of each cochlea turn, 12 SurePrint G3 Mouse Exon Microarrays (Agilent Technologies), which were spotted with 165,984 exon probes (24,547 genes), were hybridized to labeled cRNA (4 microarrays were used for each turn sample). Prior to the hybridization step, Cyanine 3-labeled cRNAs were fragmented using 25X fragmentation buffer at 60°C in a water bath for 30 min and then hybridized to a microarray slide for 17 hours at 65°C in a hybridization oven and washed using Gene Expression Wash Buffer (Agilent Technologies).

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Manufacturer protocol

Download the product protocol from Agilent Technologies for SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes) below.

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