High Pure FFPET RNA Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For better RNA yield, Store all buffers at +15 to +25°C	- To promote selective binding of RNA to the glass fibers add 0.5 volume of absolute ethanol to the lysate.

Protocol tips
- For better RNA yield, Store all buffers at +15 to +25°C
Downstream tips
- To promote selective binding of RNA to the glass fibers add 0.5 volume of absolute ethanol to the lysate.

Publication protocol

In a first step, seven RNA extraction kits (Table 1) were tested on rat renal tissue. 12 samples were analyzed for each kit: Four samples with one rat whole kidney section, four samples with two whole kidney sections and four samples with 80–101 LCM glomerular cross-sections. All sections had a thickness of 5 lm. Later, the four kits, which yielded the highest amount of RNA extracted from rat whole kidney sections were used to extract RNA from human renal tissue: Six samples were analyzed for each kit: Four samples with two human kidney biopsy sections and two samples with 80–160 LCM glomerular cross-sections. Human samples had a thickness of 10 lm. Each of the seven different kits was performed according to the manufacturer’s instructions under RNAse-free conditions. The volume of eluate was set to 25 lL in the case of the rat tissues and 15 lL in the case of the human tissue.

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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure FFPET RNA Isolation Kit below.

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